Journal article
E. Subramanian, A. Elewa, Gonçalo Brito, Anoop Kumar, Åsa Segerstolpe, Christos Karampelias, Åsa K. Björklund, R. Sandberg, K. Echeverri, W. Lui, Olov Andersson, András Simon
Developmental Cell, 2023
Developmental Cell, 2023
Semantic Scholar
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APA
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Subramanian, E., Elewa, A., Brito, G., Kumar, A., Segerstolpe, Å., Karampelias, C., … Simon, A. (2023). A small noncoding RNA links ribosome recovery and translation control to dedifferentiation during salamander limb regeneration. Developmental Cell.
Chicago/Turabian
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Subramanian, E., A. Elewa, Gonçalo Brito, Anoop Kumar, Åsa Segerstolpe, Christos Karampelias, Åsa K. Björklund, et al. “A Small Noncoding RNA Links Ribosome Recovery and Translation Control to Dedifferentiation during Salamander Limb Regeneration.” Developmental Cell (2023).
MLA
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Subramanian, E., et al. “A Small Noncoding RNA Links Ribosome Recovery and Translation Control to Dedifferentiation during Salamander Limb Regeneration.” Developmental Cell, 2023.
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@article{e2023a,
title = {A small noncoding RNA links ribosome recovery and translation control to dedifferentiation during salamander limb regeneration.},
year = {2023},
journal = {Developmental Cell},
author = {Subramanian, E. and Elewa, A. and Brito, Gonçalo and Kumar, Anoop and Segerstolpe, Åsa and Karampelias, Christos and Björklund, Åsa K. and Sandberg, R. and Echeverri, K. and Lui, W. and Andersson, Olov and Simon, András}
}
Abstract
Building a blastema from the stump is a key step of salamander limb regeneration. Stump-derived cells temporarily suspend their identity as they contribute to the blastema by a process generally referred to as dedifferentiation. Here, we provide evidence for a mechanism that involves an active inhibition of protein synthesis during blastema formation and growth. Relieving this inhibition results in a higher number of cycling cells and enhances the pace of limb regeneration. By small RNA profiling and fate mapping of skeletal muscle progeny as a cellular model for dedifferentiation, we find that the downregulation of miR-10b-5p is critical for rebooting the translation machinery. miR-10b-5p targets ribosomal mRNAs, and its artificial upregulation causes decreased blastema cell proliferation, reduction in transcripts that encode ribosomal subunits, diminished nascent protein synthesis, and retardation of limb regeneration. Taken together, our data identify a link between miRNA regulation, ribosome biogenesis, and protein synthesis during newt limb regeneration.