Journal article
Christos Karampelias, Habib Rezanejad, Mandy Rosko, Likun Duan, Jing Lu, L. Pazzagli, P. Bertolino, C. Cesta, Xiaojing Liu, G. Korbutt, Olov Andersson
Nature Communications, 2021
Nature Communications, 2021
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APA
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Karampelias, C., Rezanejad, H., Rosko, M., Duan, L., Lu, J., Pazzagli, L., … Andersson, O. (2021). Reinforcing one-carbon metabolism via folic acid/Folr1 promotes β-cell differentiation. Nature Communications.
Chicago/Turabian
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Karampelias, Christos, Habib Rezanejad, Mandy Rosko, Likun Duan, Jing Lu, L. Pazzagli, P. Bertolino, et al. “Reinforcing One-Carbon Metabolism via Folic Acid/Folr1 Promotes β-Cell Differentiation.” Nature Communications (2021).
MLA
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Karampelias, Christos, et al. “Reinforcing One-Carbon Metabolism via Folic Acid/Folr1 Promotes β-Cell Differentiation.” Nature Communications, 2021.
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@article{christos2021a,
title = {Reinforcing one-carbon metabolism via folic acid/Folr1 promotes β-cell differentiation},
year = {2021},
journal = {Nature Communications},
author = {Karampelias, Christos and Rezanejad, Habib and Rosko, Mandy and Duan, Likun and Lu, Jing and Pazzagli, L. and Bertolino, P. and Cesta, C. and Liu, Xiaojing and Korbutt, G. and Andersson, Olov}
}
Abstract
Diabetes can be caused by an insufficiency in β-cell mass. Here, we performed a genetic screen in a zebrafish model of β-cell loss to identify pathways promoting β-cell regeneration. We found that both folate receptor 1 (folr1) overexpression and treatment with folinic acid, stimulated β-cell differentiation in zebrafish. Treatment with folinic acid also stimulated β-cell differentiation in cultures of neonatal pig islets, showing that the effect could be translated to a mammalian system. In both zebrafish and neonatal pig islets, the increased β-cell differentiation originated from ductal cells. Mechanistically, comparative metabolomic analysis of zebrafish with/without β-cell ablation and with/without folinic acid treatment indicated β-cell regeneration could be attributed to changes in the pyrimidine, carnitine, and serine pathways. Overall, our results suggest evolutionarily conserved and previously unknown roles for folic acid and one-carbon metabolism in the generation of β-cells. Regeneration of insulin-producing beta-cells may become a future alternative treatment of diabetes. Here the authors report a genetic screen in a zebrafish model that mimics the loss of beta-cells in diabetes, and identified that the folate receptor Folr1 or folinic acid treatment can stimulate beta-cell regeneration.